BD Horizon™ 可固定活力染色剂 620 564996

BD Horizon™ Fixable Viability Stain 620 564996

抗体详情

BD Horizon™ Fixable Viability Stain 620 (FVS620) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion and covalent binding of the dye to the higher levels of total cellular amines when compared to living cells with impermeable plasma membranes.  Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their FVS620 fluorescence intensities 10-20 fold over that of viable cells. The FVS620-labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

BD Horizon™ Fixable Viability Stain 620 is best excited by the Yellow-Green laser (with an excitation maximum of 523 nm), but is also well-excited by the Blue laser. It has a fluorescence emission maximum of 617 nm.

BD Horizon™ 可固定活力染色剂 620 564996

BD Horizon™ Fixable Viability Stain 620 564996